Control of biofilm-producing Aeromonas bacteria in the water tanks and drinkers of broiler poultry farms using chitosan nanoparticle-based coating thyme oil

In a drinking water distribution system, biofilm-producing bacteria are considered an alarm bell for increased emergence of waterborne pathogens. This study aims to monitor the prevalence biofilm-forming Aeromonas species in systems different broiler chicken farms. The antimicrobial activity thyme essential oil (TEO), nano-emulsion (TEO-N), chitosan (CS), nanoparticles (CS-NPs), and both CS CS-NP-based coating TEO against spp.was evaluated using broth microdilution agar well diffusion assay. overall rate spp. was 49.3% (74.0/150). highest isolates noted drinkers tanks 75.0% (30/40) 62.5% (25/40), respectively) followed by feedstuff 40.0% (12/30). contrast, percentage hydrophila 70.0% (14/20) caviae 30.0% (6/20). fatal effect CS-NPs all isolated achieved 100% at 1.5 2.0 µg/mL. Moreover, (CS-NPs/TEO) verified lethal on A. ratio 1:1 1:0.75 conclusion, main source system unhygienic status that allowed biofilm produce due aggregation inner surface equipment. Both CS-NPs/TEO could be applied as sanitizer and/or disinfectant control.